Spatially selective vagus nerve stimulation (sVNS) offers a promising approach for addressing heart disease with enhanced precision. Spatially-selective VNS offers a promising solution to induce cardioprotection while mitigating off-target effects by specifically targeting pre-ganglionic parasympathetic efferent cardiac fibers. This study examined the spatial organization of cardiac fibers in pig vagus nerves to enable more precise nerve stimulation therapy. Using in vivo selective stimulation and imaging techniques, we found significant spatial separation between cardiac afferent and cardiac efferent fibres at the mid-cervical level with opposite locations across nerve cross-sections. Cardiac efferent regions proved exclusive, positioned near recurrent laryngeal areas, while afferent fibers clustered near pulmonary fibers. The selective stimulation achieved heart rate reductions without triggering cardiac afferent reflexes. These anatomical discoveries suggest targeted vagus nerve stimulation could provide therapeutic cardiac benefits while minimizing unwanted off-target effects.